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It has a healthy and positive impact in your body. How it works: Situation: besides walking, there are two options to go up:Phenyl-alpha-pyrone derivatives from Helicostylis auriculata protect against oxidative stress in skin cells of zebrafish (Danio rerio).
A new phenyl-alpha-pyrone was isolated from the whole plant of Helicostylis auriculata, together with eleven known analogues. Their structures were determined by spectroscopic analysis. All compounds were evaluated for their protective activity against oxidative stress induced by tert-butylhydroperoxide in Chinese hamster ovary (CHO) cells and zebrafish skin cells. None of the phenyl-alpha-pyrone derivatives from H. auriculata presented cytotoxicity towards CHO cells. The greatest protective activity was found for the 2- and 3-hydroxy metabolites, which exhibited half-maximal effective concentrations (EC50) between 2.2-3.5 and 11.8-26.6 microM, respectively. However, the parent compound had an EC50 of 83.9 microM. Moreover, in vivo tests with zebrafish demonstrated that all the derivatives were also able to protect against the stress caused by oxidative species, with EC50s between 4.7 and 7.1 microM. Among the most active derivatives in zebrafish skin cells, the 2-hydroxy product was the most potent, with an EC50 of 8.6 microM, significantly more effective than the reference product (a mixture of vitamins A, C and E) with an EC50 of 50.4 microM. The results obtained from the zebrafish skin cell system were also confirmed in the CHO cell model. SUPREME COURT OF THE STATE OF NEW YORK
Appellate Division, Fourth Judicial Department
385
KA 13-00658
PRESENT: SMITH, J.P., CARNI, LINDLEY, SCONIERS, AND WHALEN, JJ.
THE PEOPLE OF THE STATE OF NEW YORK, RESPONDENT,
V MEMORANDUM AND
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