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Microglial cells are activated and produce a variety of inflammatory mediators in response to a variety of stimuli. Here we examined the effect of lipopolysaccharide (LPS) in mouse microglial cell line BV2. As shown by Western blot analysis, LPS dramatically induced alpha-synuclein (SNCA) expression in BV2 cells, but the expression levels of other genes associated with neuroinflammation, such as cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), and inducible nitric oxide synthase (iNOS) were lower. LPS induced the expression of SNCA as early as 3 h after stimulation, with the highest levels at 24 h. It also induced significant increases in the nuclear NF-kappa B p65 subunit, but the levels of cytoplasmic NF-kappa B p50 subunit were unchanged. LPS-induced alpha-synuclein expression was also confirmed by immunofluorescence and immunocytochemistry. These results suggest that alpha-synuclein might be a potential link between inflammation and the pathological process of neurodegenerative diseases.CBN ANNOUNCES 2008 ELECTIONS
BARRIE, ONTARIO, March 12, 2008
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The 2008 CANA workshop entitled “How a Church Chooses a Pastor” is scheduled for April 22-25 at the Full Gospel Church in Barrie, Ontario. The workshop is open to all who are interested in training for the CANA ministry. Registration for the two-day workshop is $100 and includes a two-day training, a one-day CANA train-the-trainer workshop, a Bible study, transportation, and meals.
This is a CANA ministry workshop designed for those
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