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We investigated the effects of inhalation of volatile organic compounds (VOCs) on pressor responses elicited by intranasal (i.n.) administration of arginine vasopressin (AVP) in conscious rats. Mixture of acetone and chloroform vapor containing the VOCs vapors inhibited AVP-induced pressor response in a dose-dependent manner. The inhibitory effect was abolished by pretreatment with the beta 2-adrenergic receptor antagonists, atenolol (50 mg/kg, s.c.) or ICI 118,551 (0.5 mg/kg, i.v.) and blocked by pretreatment with the alpha-adrenergic receptor antagonist prazosin (10 mg/kg, i.v.). The volatile compound-induced inhibition of AVP-induced pressor responses was not affected by ganglion blockade with the non-alpha-adrenergic ganglionic receptor blocker hexamethonium (20 mg/kg, i.v.). By contrast, the response was significantly inhibited by pretreatment with the alpha 1-adrenergic receptor antagonist prazosin (0.25 mg/kg, i.v.) or by pretreatment with the neuropeptide-Y (NPY)-Y1 receptor antagonist RC-3095 (1 mg/kg, i.v.). The combination of prazosin and alpha-methyl-p-tyrosine significantly decreased the pressor responses induced by AVP. These results suggest that inhalation of volatile compounds inhibits vasopressin-induced pressor responses in rats, probably through the coupling of G-proteins with the NPY-Y1 receptor.
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