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A new infrared spectroscopic (IR) technique has been developed to monitor the hydration behavior of proteins in water solution. The essential idea of the new method is to replace the real protein under investigation by a model protein with comparable composition, conformation, pH and ionic strength, which has an identical (or close) infrared spectrum and behaves similarly under scanning conditions. Lysozyme, a biologically important protein, was used as a model system to test the technique. New Proteins for IR Spectroscopy (PRINS) is a differential scanning calorimetry (DSC) test method that replaces lysozyme by a model protein with an identical (or close) infrared spectrum. The new IR method measures the change in the hydration level of lysozyme (i.e., the hydration of the protein in water) relative to that of the model protein. The new IR technique provides a new powerful method for hydration determination of proteins, in contrast to other techniques.President Obama announced Thursday that the Defense Department and intelligence community will launch a comprehensive review of the collection of DNA from people convicted of federal crimes as part of what he said was the most “proactive” review of the collection of biometric data since the 1979 National Security Act.
The review will cover “any personally identifiable information – DNA or other biometric information –” collected in such cases. The review, starting in the next 60 days, will be overseen by an interagency group of privacy and civil liberties experts, including the White House Privacy and Civil Liberties Office.
“We are at a moment in our history when there are new capabilities,” said Obama, who told the annual Aspen Security Forum in Colorado, “that can identify, in a non-
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The efficacy of the transplantation of insulin-producing human islet cells into nude mice and the influence of the portal vein and portal vein-portal vein route on islet cells grafting was investigated in this study. After the experimental groups of nude mice were given 2 or 3 x 10(4) islet cells via portal vein or portal vein-portal vein route, the grafts were monitored for 4 weeks by the reverse hemagglutination (RH) test. After 4 weeks, all nude mice transplanted with the 3 x 10(4) islet cells showed the positive grafts by RH test. However, only about 50% of mice gave positive grafts when the transplantation dose of islet cells was 2 x 10(4). The proportion of positive grafts was correlated with the number of islet cells grafted. The grafts obtained via portal vein route were larger than those of portal vein-portal vein route. Thus, it was suggested that portal vein-portal vein route was not suitable for islet cell transplantation.Gait abnormalities in the treatment of childhood idiopathic hip disorders.
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