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We report the application of a microfluidic continuous-flow (CF) column as a novel tool to efficiently study the effect of small molecules on growth factor signaling pathways. The technology provides a continuous-flow microfluidic platform that integrates a deformable microfluidic device with an optical sensor for continuous monitoring of cell growth. This technology was used to probe the inhibition by colchicine of the TGF-beta and IGF-I signaling pathways in a time-dependent manner. The resulting outputs offer an effective tool to screen small molecules based on their ability to inhibit growth factor signaling and affect cell growth and survival.
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Electroerosion is a method of cutting material by means of a head that is provided with a sharp edge of a material. The electroerosion method has been used for making iron castings and cut grooves in steel plate. In the electroerosion method, a die assembly is used. A die assembly is a kind of an electroerosion head. The die assembly includes an electroerosion head and a cutting plate. The electroerosion head is provided with a sharp edge, and the cutting plate is clamped on a workpiece. When the electroerosion head is provided with a voltage, an electric current flows along the sharp edge to the workpiece. Therefore, the material of the workpiece is cut by the electric discharge. The die assembly is moved relative to a workpiece to form a cut groove in the workpiece.
Since electroerosion involves the discharge of a large amount of energy, various methods for preventing excessive energy from being delivered to the workpiece have been proposed. For example, Japanese Examined Utility Model Application Publication No. 4-39553 (Japanese Unexamined Utility Model Application Publication No. 3-107148) discloses an electroerosion apparatus in which the intensity of an electric current is adjusted with respect to the size of a cutting groove to be formed on a workpiece. When the electroerosion apparatus cuts the workpiece, the depth and width of the cut groove can be adjusted with respect to the size of the die assembly. In this electroerosion apparatus, the electric current is adjusted on a pulse base. That is, the apparatus causes the workpiece to be cut by a pulse of an electric current.
The method of adjusting an electric current with respect to the depth and width of a cut groove is effective in reducing the occurrence of excessive energy. However, this method
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