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A novel method for the determination of thyrotropin-releasing hormone (TRH) in bovine milk was developed. The method is based on homogeneous enzyme immunoassay (EIA) using a specific polyclonal anti-TRH antibody. The immunoassay developed was characterised in terms of selectivity, specificity, linearity, recovery, limit of detection (LOD) and limit of quantification (LOQ). Following cleanup with mixed-mode adsorbents, TRH was identified and quantified using liquid chromatography tandem mass spectrometry (LC-MS/MS). The optimized LC-MS/MS method was validated and its reproducibility and ruggedness were also evaluated. The validated method was applied to a set of control and milk samples, including raw milk and milk supplemented with TRH. The results obtained, together with a discussion of the potential benefits and drawbacks of this immunoassay, are reported.A variety of different optical application systems and methods are known in the art for generating and projecting a beam of light into an object, capturing reflected light from the object, and/or detecting light that has passed through the object. These systems and methods are used in a variety of applications, such as optical displays, optical recording, and other optical imaging applications. A source of light (or beams of light) is commonly used to illuminate an object in these optical applications. The light source may be a point source, or alternatively, the light source may comprise one or more light emitting elements (e.g., light emitting diodes) in an array. Generally, the light source is disposed at a distance from the object and provides a beam of light to the object. Reflected light that is reflected by the object and/or light that has passed through the object are received by a detector. The detector may be an array of detector elements. Typically, a two-dimensional array of detector elements is used as the detector. The output signal of the detector is indicative of the brightness and/or color of the reflected light received by the detector, and
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