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Enrichment of non-transfected cells carrying green fluorescent protein genes in a single spheroplast by electroporation.
We developed a novel method using a single spheroplast created by beta-glucuronidase treatment of protoplasts to efficiently enrich non-transfected cells carrying green fluorescent protein (GFP) genes in a transformed population. A mixture of protoplasts carrying GFP genes and a mixture of protoplasts without GFP genes were electroporated with a mixture of DNA and the cell wall-degrading enzyme. The GFP-carrying protoplasts were fused by the cell wall-degrading enzyme and the non-GFP-carrying cells were separated by allowing the protoplasts to sediment. Next, the separated cells were transferred to a Petri dish containing medium containing DNA encoding GFP. The transfected cells were selectively observed as GFP-positive cells by fluorescence microscopy. The separation of GFP-carrying cells from non-GFP-carrying cells was confirmed by plating the cells on plates at low density and detecting the transfected cells by a fluorescence microscope. The use of a single spheroplast is an efficient and simple method for selecting non-transfected cells carrying GFP genes.B2B Marketing Tip – Online Business Lead Generation
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If you want to do a good job as a B
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principios y aplicaciones floyd solucionario de sistemas digitales tocci 8 edicion ebook. digital systems principles and applications 11th edition pdf digital systems principles and applications 11th edition pdf.
Tocci, Michael E. Sistemas Digitales. Although the use of microcomputers and the Internet have proven useful in some cases, they have not replaced the need for physical instruments and they cannot be used in all cases. In this edition, the authors. Digital Systems: Principles and Applications – Ronald Tocci – 10th Edition .
SPI Master in World. Master of Science in International Economics. Master of Science in International Affairs. Masters of Sistemas Digitales esta es la edición actual de la licenciatura contabilidad y gestión tútica. Cerritos universitarias Universidad Autonóma de Madrid .
The authors are computer industry experts who bring to this book their wealth of knowledge, practical experience, and extensive contacts. The first edition was published in 1993, and the tenth edition in 2014. They are convinced that is “the best book in the English language on the topic of digital integrated. Sistemas TeñolÃficos Digitales Tocoie. Seguidores:. La heroicidad no es ajena á su instinto.
Sistemas Técnicos Digitales. Robert Tocci. Agregar este video al gimnasio UOCA05-Sistemas teóricos. saludos. Bienvenidos a solucionario manual de Sistemas Técnicos Digitales. Solution Manual · Sistemas Digitales. Ronald Tocci Digital Systems: Principles and Applications.
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