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strand 7 full version crackSensitization of the slow inward current by agmatine in guinea pig ventricular myocytes.
The effects of agmatine, an endogenous inhibitor of cell membrane guanine nucleotide binding proteins on the whole-cell outward currents were examined using the patch-clamp technique in guinea pig ventricular myocytes. Agmatine (30-100 microM) induced a small outward current with little shift in the holding current. The time-dependent outward current elicited by agmatine was found to be markedly inhibited by 0.1 mM 8-chloroadenosine 3′,5′-monophosphate (8-Cl-cAMP), whereas the time-dependent inward current induced by agmatine was not inhibited by 8-Cl-cAMP. 8-Cl-cAMP (0.1 mM) also failed to inhibit the outward current induced by agmatine (100 microM). The outward current induced by agmatine (100 microM) was also reduced by pretreatment with agmatine (30-100 microM) for 5 min and the time-dependent outward current elicited by agmatine (100 microM) during pretreatment with agmatine (100 microM) was further inhibited by 8-Cl-cAMP (0.1 mM) or hemicholinium-3 (10 microM). The results indicate that the agmatine-induced outward current is dependent on the guanine nucleotide regulatory protein, but not the adenylate cyclase-cyclic AMP pathway. This is the first report demonstrating that agmatine directly hyperpolarizes the membrane and inhibits the slow inward current (Isi) in guinea pig ventricular myocytes.Long-term results of minimally invasive endoscopic resection of esophageal gastrointestinal stromal tumors (GISTs).
To report the long-term results of endoscopic treatment for esophageal gastrointestinal stromal tumor (ESGIST). We conducted a retrospective analysis of 7 patients who underwent minimally invasive endoscopic treatment for ESGIST. Patients were followed up for a median period of 42.7 months after initial endoscopic therapy. Initial successful en-bloc endoscopic resection was obtained in 6 of 7 patients. Recurrence was observed in 2 (33%) patients after a median interval of 15.5 months. Two (33%) patients died after a median interval
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