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The effect of the plasticizer, di(2-ethylhexyl)phthalate (DEHP) on the reaction catalyzed by the Ca2+-ATPase has been studied in the frog skeletal muscle sarcoplasmic reticulum. A dose-dependent inhibition of the hydrolysis of ATP in the presence of Ca2+ was observed; the inhibition being expressed by a decrease in the initial rate of Ca2+ transport. The Ca2+ efflux is not affected by DEHP. The Ca2+ uptake is inhibited by 20-35% upon exposure to DEHP concentrations as low as 1 microM. The inhibition of Ca2+ uptake produced by DEHP could not be reversed by repletion of the sarcoplasmic reticulum with Ca2+ in the presence of the inhibitor. The data indicate that DEHP does not act through a direct inhibition of the Ca2+-transport ATPase, but that it interferes with the coupling between Ca2+ transport and ATP hydrolysis. DEHP does not release Ca2+ from the internal Ca2+ pools of the sarcoplasmic reticulum. The reduced Ca2+ sequestered in the intracellular Ca2+ pool in presence of DEHP, however, indicates the existence of a Ca2+ “leak” from the sarcoplasmic reticulum membrane into the cytosol.Jorge Santos Dumont was the first to reach space—and the Brazilian space agency FAP believes that it has just found another historic aviation record. This time in
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